Journal: Molecules

Article Title: Gellan Gum/Alginate Films Containing Biogenic uva ursi Silver Nanoparticles: Analytical Characterization and Antiviral Activity Against HSV-1

doi: 10.3390/molecules31091459

Figure Lengend Snippet: Nanoparticle tracking analysis (NTA) of AgNPs in a 1 mL sample. ( A ) Hydrodynamic diameter size per concentration, obtained using the finite track length adjustment (FTLA) algorithm, with triplicate measurements of the AgNPs sample. ( B ) Average of the three measurements. All measurements were run with non-diluted samples. ( C ) Relative particle concentration (particles/mL) is plotted as a function of particle size (nm). Three independent measurements are shown (Replicate 1–3; colored lines), together with the mean distribution (black line) and the shaded area representing ± standard error of the mean (SEM). The particle size distribution exhibits a unimodal profile, with the majority of particles in the 30–80 nm range. The data presented represent a normalized and scaled distribution derived from particle counts and do not correspond to absolute particle concentration values. The x-axis was rescaled to 0–100 nm to improve visualization of the size distribution.

Article Snippet: The concentration of uva-ursi AgNPs was determined using a NanoSight NS300 NTA instrument (Malvern Panalytical, Great Malvern, UK).

Techniques: Concentration Assay, Derivative Assay

Journal: Molecules

Article Title: Gellan Gum/Alginate Films Containing Biogenic uva ursi Silver Nanoparticles: Analytical Characterization and Antiviral Activity Against HSV-1

doi: 10.3390/molecules31091459

Figure Lengend Snippet: Morphological characterization of AgNPs prepared by uva-ursi by transmission electron microscopy ( A – D ). Size distribution histogram ( E ) (R 2 = 0.89081) of AgNPs.

Article Snippet: The concentration of uva-ursi AgNPs was determined using a NanoSight NS300 NTA instrument (Malvern Panalytical, Great Malvern, UK).

Techniques: Transmission Assay, Electron Microscopy

Journal: Molecules

Article Title: Gellan Gum/Alginate Films Containing Biogenic uva ursi Silver Nanoparticles: Analytical Characterization and Antiviral Activity Against HSV-1

doi: 10.3390/molecules31091459

Figure Lengend Snippet: Evaluation of the two AgNP-containing films, GG 1.6 -NaALG 0.4 /AgNPs ( A ) and GG 1.2 -NaALG 0.8 /AgNPs ( B ), and of AgNP ( C ) cytotoxicity. Cytotoxicity was assessed in Vero-76 cells using an MTT assay after 24 h of treatment with the sample suspension. Blank films, without AgNPs, referred to as GG 1.6 -NaALG 0.4 and GG 1.2 -NaALG 0.8 , were tested non-diluted. Data are reported as mean ± SD. CTR−: untreated cells; CTR+: 100% DMSO. Statistical analysis was performed using Dunnett’s multiple comparisons test: **** p -value < 0.0001; *** p -value = 0.0004; ** p -value = 0.0042; * p -value = 0.0358; ns p -value > 0.05.

Article Snippet: The concentration of uva-ursi AgNPs was determined using a NanoSight NS300 NTA instrument (Malvern Panalytical, Great Malvern, UK).

Techniques: MTT Assay, Suspension

Journal: Molecules

Article Title: Gellan Gum/Alginate Films Containing Biogenic uva ursi Silver Nanoparticles: Analytical Characterization and Antiviral Activity Against HSV-1

doi: 10.3390/molecules31091459

Figure Lengend Snippet: Time-shift assays of the two AgNP-containing films ( A , C ), GG 1.6 -NaALG 0.4 /AgNPs and GG 1.2 -NaALG 0.8 /AgNPs, and of AgNPs ( B , D ) to evaluate their potential antiviral effect against HSV-1. Two different plaque reduction assays are shown. ( A , B ) Co-treatment assay; ( C , D ) virus pre-treatment assay. The untreated cells represented the negative control (CTR−). Melittin (5 µM) was used as a positive control (CTR+) in the co-treatment and virus pre-treatment. Data are reported as mean ± standard deviation (SD) of three independent experiments. **** p -value < 0.0001; *** p -value < 0.0006; ns p -value > 0.05.

Article Snippet: The concentration of uva-ursi AgNPs was determined using a NanoSight NS300 NTA instrument (Malvern Panalytical, Great Malvern, UK).

Techniques: Virus, Negative Control, Positive Control, Standard Deviation

Journal: Molecules

Article Title: Gellan Gum/Alginate Films Containing Biogenic uva ursi Silver Nanoparticles: Analytical Characterization and Antiviral Activity Against HSV-1

doi: 10.3390/molecules31091459

Figure Lengend Snippet: Temperature-shift assays of the two AgNP-containing films ( A , C ), GG 1.6 -NaALG 0.4 /AgNPs and GG 1.2 -NaALG 0.8 /AgNPs, and of AgNPs ( B , D ) to evaluate their potential antiviral effect against HSV-1. Two different plaque reduction assays are shown. ( A , B ) Attachment assay; ( C , D ) entry assay. Untreated cells served as the negative control (CTR−), while heparin (1 mg/mL) was used as the positive control (CTR+) in both assays. Data are reported as mean ± standard deviation (SD) of three independent experiments. Statistical significance was determined as follows: **** p -value < 0.0001; *** p -value < 0.0006; ns p -value > 0.05.

Article Snippet: The concentration of uva-ursi AgNPs was determined using a NanoSight NS300 NTA instrument (Malvern Panalytical, Great Malvern, UK).

Techniques: Negative Control, Positive Control, Standard Deviation

Journal: Molecules

Article Title: Gellan Gum/Alginate Films Containing Biogenic uva ursi Silver Nanoparticles: Analytical Characterization and Antiviral Activity Against HSV-1

doi: 10.3390/molecules31091459

Figure Lengend Snippet: Schematic representation of the uva-ursi AgNP preparation steps and the UV-Vis absorption spectra of AgNO 3 solution (light blue line), Arctostaphylos uva-ursi extract (green line), and uva-ursi AgNPs (fuchsia line).

Article Snippet: The concentration of uva-ursi AgNPs was determined using a NanoSight NS300 NTA instrument (Malvern Panalytical, Great Malvern, UK).

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Photoprotection of a novel resveratrol analogue: insights from 2D, 3D, and endothelialized bioprinted human skin models

doi: 10.3389/fphar.2026.1756483

Figure Lengend Snippet: UVA-induced intracellular ROS production in the models: (a) Fibro: reconstructed human skin model (without HUVEC); (b) Fibro + HUVEC: reconstructed human skin model containing HUVEC; (c) Bioprinted: endothelialized bioprinted human skin model; (d) Fibro: reconstructed human skin model (without HUVEC) exposed to the sun simulator. Results are expressed as the percentage of fluorescence in comparison to the NT + UV. Untreated non-irradiated control (NT -UV); Untreated irradiated control (NT + UV); Trz-HBH 3 : resveratrol analogue obtained from a molecular triplication process (200 μg/mL); Vehicle: sesame oil; UV lamp: UVA-emitting lamp. Results are expressed as mean ± standard error of the mean from three independent experiments. Different letters indicate statistically different means ( p < 0.05), according to one-way ANOVA followed by Tukey’s post hoc test. Statistical analysis was performed using Minitab (version 18), with n = 3 of independent experiments and a significance level set at p < 0.05. Figures were generated using GraphPad Prism (version 9.0).

Article Snippet: Then, the tissues were submitted (+UV) or not (-UV) to 10 J/cm 2 of UVA radiation emitted by a sun simulator (SOL-500 Dr Honle AG, Planegg, Germany) or an UVA lamp (CAMAG, 960914).

Techniques: Fluorescence, Comparison, Irradiation, Control, Generated